《植物生理学报》 2018, 54(5): 745-754

ERF转录因子家族在甜菜块根发育中的功能分析

曹国丽, 张永丰, 孙亚卿, 李国龙, 张少英^*

内蒙古农业大学甜菜生理研究所, 呼和浩特010018

通信作者：张少英；E-mail: syzh36@aliyun.com

摘　要：

本研究以AP2/ERF保守结构域序列为检索序列在甜菜(Beta vulgaris)基因组数据中搜索并鉴定BvERF转录因子家族成员。通过系统分析, 发现甜菜基因组中共有68个BvERF家族基因, 系统进化分析将它们分为10个组。BvERF家族中15个成员与甜菜块根转入快速生长阶段有关, 其中Bv_ammr可能与甜菜丰产性能有关, Bv_ignp、Bv_sqfr和Bv_khde与苗龄113 d时丰产型品种生长速率较高有关, Bv_wnjc可能与块根生长速率负相关, Bv_fefj基因与高糖型品系具有更多的维管束环有关。研究结果为进一步明确BvERF转录因子调控甜菜块根发育的分子机制提供参考。

关键词：甜菜; 块根发育; BvERF

收稿：2018-02-05   修定：2018-03-27

资助：国家自然科学基金(31760416)和现代农业产业技术体系项目(CARS-170201)。

Functional analysis of ERF transcription factor family in taproot development of sugar beet

CAO Guo-Li, ZHANG Yong-Feng, SUN Ya-Qing, LI Guo-Long, ZHANG Shao-Ying^*

Sugar Beet Physiological Research Institute, Inner Mongolia Agricultural University, Hohhot 010018, China

Corresponding author: ZHANG Shao-Ying; E-mail: syzh36@aliyun.com

Abstract:

In this study, in order to identify BvERF transcription factor family, the sequence of AP2/ERF conserved domain was used as baits to search in sugar beet (Beta vulgaris) genome. According to phylogenetic analysis, conservative motif analysis and the expression pattern profiling of BvERF, 68 BvERFs were identified, and classified into 10 groups. A total of 15 members of BvERF family were involved in taproot turning to rapid growth. Among them, Bv_ammr may be related to the high yield characteristic of sugar beet. Bv_ignp, Bv_sqfr and Bv_khde were related to that the taproot of high taproot yield cultivar grew more rapidly than the taproot of high sucrose content cultivar. Bv_wnjc may be negatively relevant to the growth rate of taproot. Bv_fefj may be involved in taproot with more vascular ring in high sucrose content line. These results provide a reference for the further study of the molecular mechanism that was involved in how BvERF regulates the taproot development of sugar beet.

Key words: sugar beet; taproot development; BvERF